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CRISPR double nickase-based amplification composition, system, and method

机译:基于CRISPR双酸氨基酶的扩增组合物,系统和方法

摘要

Embodiments disclosed herein provide a powerful CRISPR-based nucleic acid amplification method and system using an RNA targeting effector. Embodiments disclosed herein are capable of amplifying both double-stranded and single-stranded nucleic acid targets. In addition, embodiments disclosed herein can be combined with various detection platforms, such as CRISPR-SHERLOCK, to achieve detection and diagnosis in atomolar sensitivity. This embodiment is useful in a number of scenarios in human health, including, for example, virus, detection, bacterial strain type analysis, sensitive genotyping, and detection of disease-associated cell free DNA.
机译:本文公开的实施方案提供了使用RNA靶向效应器的基于强大的基于核酸核酸扩增方法和系统。本文公开的实施方案能够扩增双链和单链核酸靶标。另外,本文公开的实施方案可以与各种检测平台组合,例如CRISPR-Sherlock,以实现热摩尔敏感性的检测和诊断。该实施方案可用于人类健康中的许多情景,包括例如病毒,检测,细菌菌株类型分析,敏感基因分型和疾病相关的细胞自由DNA的检测。

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