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CRISPR double nickase-based amplification composition, system, and method
CRISPR double nickase-based amplification composition, system, and method
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机译:基于CRISPR双酸氨基酶的扩增组合物,系统和方法
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摘要
Embodiments disclosed herein provide a powerful CRISPR-based nucleic acid amplification method and system using an RNA targeting effector. Embodiments disclosed herein are capable of amplifying both double-stranded and single-stranded nucleic acid targets. In addition, embodiments disclosed herein can be combined with various detection platforms, such as CRISPR-SHERLOCK, to achieve detection and diagnosis in atomolar sensitivity. This embodiment is useful in a number of scenarios in human health, including, for example, virus, detection, bacterial strain type analysis, sensitive genotyping, and detection of disease-associated cell free DNA.
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