A method of and incubator for preparing bacterial cultures, especially bacterial cultures for producing fermented milk products such as yogurt by inoculation and heat treatment of a nutrient solution with an external culture in a sterilized incubator, according to which the incubator is charged and discharged and specimens are obtained by means of a cannula which extends through a narrow aperture in the upper part of the incubator and which is covered by a heat barrier, e.g. a flame barrier or a hot gas barrier, during charging and discharging and is closed in a sterile manner with an air-permeable sterilization plug after the cannula has been removed.
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