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PRODUCTION OF MONOCLONAL ANTIBODY AGAINST RICE DWARF VIRUS

机译:水稻矮病毒单克隆抗体的制备。

摘要

PURPOSE:A cell hybrid is cloned and the clone which produces an antibody showing specificity to rice dwarf virus is selected and cultured to expand the kinds of monoclonal antibodies to plant viruses. CONSTITUTION:An animal is immunized with rice dwarf virus and the resistance productive cell from the animal is fused with myeloma cell to form fused cell hybrid and the cell is cloned. Then, the clon producing antibody showing specificity to rice dwarf virus is selected and cultured to obtain monoclonal antibody to rice dwarf virus. The fused hybrid is obtained by fusing the cell producing antibody to rice dwarf virus from the animal with myeloma cell.
机译:目的:克隆细胞杂种,选择产生对水稻矮化病毒具有特异性的抗体的克隆,并进行培养,以扩大针对植物病毒的单克隆抗体的种类。组成:一种动物用稻矮病毒进行免疫,并将该动物的耐药性生产细胞与骨髓瘤细胞融合,形成融合的细胞杂种,并克隆该细胞。然后,选择对水稻矮化病毒具有特异性的产生克隆的抗体,并对其进行培养,以获得针对水稻矮化病毒的单克隆抗体。通过将来自动物的水稻矮化病毒的细胞产生抗体与骨髓瘤细胞融合而获得融合的杂种。

著录项

  • 公开/公告号JPS6174594A

    专利类型

  • 公开/公告日1986-04-16

    原文格式PDF

  • 申请/专利权人 KURARAY CO LTD;

    申请/专利号JP19840198852

  • 申请日1984-09-21

  • 分类号G01N33/569;A61K39/395;C07K14/005;C07K14/195;C07K16/00;C07K16/08;C12N15/02;C12P21/08;C12R1/91;G01N33/577;

  • 国家 JP

  • 入库时间 2022-08-22 07:45:58

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