NOVEL 2'-5' OLIGOADENYLIC ACID COMPOUND, PREPARATION THEREOF AND ANTIGEN FOR 125I-LABELING COMPOSED OF SAME USED FOR 2'5' OLIGOADENYLIC ACID MEASUREMENT BY RADIOIMMUNOASSAY

摘要

NEW MATERIAL:Methyl ester of 5'-triphospho (adenylyl 2'-5')2 adenosine-beta- alanyltyrosine, expressed by formula I. USE:An antigen for labeling with 125I, used to measure 2'-5' adenylic acid with RIA (radioimmunoassay). PREPARATION:5'-Monophospho (adenylyl 2'-5')2 adenosine expressed by formula II is oxidized with an oxidizing agent (example; periodic acid, etc.) and further reacted with methyl ester of beta-alanyltyrosine in DMF (dimethyl formamide) solvent in the presence of sodium hydroxide to obtain methyl ester of 5'- monophospho (adenylyl 2'-5')2 adenosine-beta-alanyltyrosine. The resultant compound is then reacted with N,N'-carbonyldiimidazole in a solvent such as DMF in the presence of triethylamine and tri-n-octylamine, and further reacted with pyrophosphoric acid. Labeling is carried out in 0.05M phosphoric acid buffer solution (7.5pH) using the 125I by chloramine T method.