PREPARATION OF (2'-5') OLIGOADENYLIC ACID

摘要

PURPOSE:To obtain the titled compound having the physiological activity in short steps without protecting the functional groups, by dissolving adenosine in an aprotic polar solvent, reacting the adenosine with a specific phosphine derivative, oxidizing the resultant phosphorous ester bonds, and selectively cleaving the ester bond at the 3'-position. CONSTITUTION:Adenosine is dissolved in an aprotic polar solvent, e.g. N,N- dimethylformamide, or a solvent containing the same essentially and then reacted with a phosphine derivative, e.g. tri-(imidazol-1-yl) phosphine, derived from a cyclic compound having an imino group in the ring, e.g. imidazole, to give an oligomer having phosphorous ester bonds at the 2'- and 3'-positions of one adenosine and 5'-position of another adenosine. The resultant oligomer is then reacted with an oxidizing agent and water to oxidize the phosphorous ester bonds and cleave the ester bond at the 3'-position selectively and give the aimed compound.