METHOD FOR THE PREPARATION OF OXIDIZED LIPOPROTEINS, METHOD FOR THE PREPARATION OF LOW DENSITY OXIDATED LIPOPROTEINS, COMPOSITION OF LOW DENSITY OXIDATED LIPOPROTEIN AND APPLIANCE FOR LIPOPROTEIN OXIDATION

摘要

Methods and apparatuses are disclosed for preparing novel oxidized lipoprotein compositions. Peroxides are capable of generating free-radicals which, in turn, can oxidize lipoproteins in vitro or in vivo. It has been discovered that hydrogen peroxide itself or in conjunction with certain enzymes such as peroxidase or lipoxidase can oxidize lipoproteins. Peroxidized low density lipoproteins are the most preferred composition. Oxidized lipoproteins exhibit a cytotoxic effect to which diseased cells which are more susceptible than healthy cells. Thus, oxidized lipoproteins may be useful in treating many disease states. The extent of lipoprotein peroxidation is measured by obtaining a C-13 NMR spectrum of the oxidized lipoprotein solution.