PROCEDURE FOR THE PRODUCTION BY FERMENTATION AND ISOLATION OF CYCLOSPORINE A, AND NEW STRAINS FORMING CYCLOSPORIN.

摘要

PROCEDURE FOR THE FERMENTATIVE ELABORATION AND ISOLATION OF CYCLOSPORIN A, AS WELL AS OF THE NEW CYCLOSPORIN FORMING ROOTS. THE INVENTION REFERS TO THE PROCEDURE FOR THE FERMENTATIVE ELABORATION AND ISOLATION OF CYCLOSPORINE A, THROUGH THE AEROBIC FERMENTATION OF A NEW ROOT OF THE SPECIES OF FUNGI SESQUICILLOPIS ROSARIENSIS G. ARNOLD, OR THROUGH THE MUTATION OF AND / OR SEARCH RESULTING ROOTS. BIOSYNTHESIS OCCURS IN A MEDIUM, WHICH CONTAINS AMMONIC SALTS AS THE ONLY SOURCE OF NITROGEN, AS WELL AS CITRATE TOGETHER WITH OTHER SOURCES OF CARBON. THE SUSPENSION OF THE CROP IS ADDED WITH AN AUXILIARY FILTERING AGENT, AND THEN IT IS FILTERED; THE SO-OBTAINED MIXTURE OF WET BIOMASS AND AUXILIARY FILTERING AGENT IS DRIED, EXTRACTED, WITH AN ORGANIC ACID ESTER OR OVER-CRITICAL GAS, PREFERABLY CO2. THE EXTRACT IS DEGREASED AND CHROMOTOGRAPHED IN THE SILICA AND / OR ALUMINUM OXIDE GEL BY HPLC PREPARATIONS.