Process for producing aspartase and process for producing L-aspartic acid

摘要

Disclosed are a process for producing aspartase which comprises culturing a microorganism belonging to the genus Escherichia being capable of producing aspartase in a medium until aspartase is produced and accumulated in the culture, and recovering aspartase therefrom, wherein dissolved oxygen concentration of the medium is in the range of 0 to 1 ppm on the stage that microbial growth is middle and/or late logarithmic growth phase; and a process for producing L-aspartic acid which comprises converting fumaric acid and ammonia into L-aspartic acid in an aqueous medium in the presence of an enzyme source, wherein said enzyme source is a culture produced in accordance with said aspartase-producing process, cells isolated from the culture, or processed cells thereof.