Solid support reagents for the direct synthesis of 3'-labeled polynucleotides

摘要

The compounds of the invention are exemplified by the class of diglycolate synthesis supports particularly useful as support reagents for the direct synthesis of 3'-labeled polynucleotides. Generally, the compounds of the invention have the structure CHEM where T is an acid-cleavable hydroxyl protecting group, e.g., 4,4'-dimethoxytrityl; L1 is a linker for connecting a 3'-terminal nitrogen to carbon; L2 and L3 are linkers for connecting oxygen and carbon; W is a solid support, e.g., CPG or polystyrene; L4 is a linker for connecting the solid support to nitrogen; R1 and R2 are nitrogen substituents, e.g., hydrogen, lower alkyl, nitrogen protecting group, or label; and R3 through R7 are carbon substituents, e.g., hydrogen or lower alkyl. In a first particularly preferred embodiment, the synthesis supports of the invention are exemplified by compounds having the structure CHEM where DMT is 4,4'-dimethoxytrityl and W is polystyrene. In a second particularly preferred embodiment, the synthesis supports of the invention are exemplified by compounds having the structure CHEM where DMT and W are defined above.l