Method for detecting molecules containing nucleotide mismatches and the location of these mismatches, and application to the detection of base substitutions or deletions in nucleotide sequences

摘要

Method for the detection and/or location of mutations or deletions in nucleotide sequences by creation of heteroduplexes between two types of double-stranded DNA able to form mismatches at the sites of said mutations or deletions. The heteroduplexes are detected as a result of the labeling of each type of strand by different fluorescent molecules or are screened by passage over a support which specifically retains them.