One of the juvenile hormone agonist or as a chemical ligand for control of gene expression in plants by receptor-mediated trans-activation

摘要

(57) [summary] The present invention describes a method of control gene expression in plants. Specifically, the method comprises transforming a plant with a target expression cassette encoding a target polypeptide and USP receptor expression cassette encoding a USP receptor polypeptide. By the contact to one of the juvenile hormone agonist or of the transformed plant, the expression of the target polypeptide is activated in the presence of USP receptor polypeptide. If desired receptor expression cassette obtained, the second encoding the receptor polypeptide that is different from the USP using a cassette expression receptor "second". This method is useful in the control of the important properties agronomically such various plant fertility. Method of identifying a ligand of USP was unknown also been described previously. Are identified by contacting a plant cell transformed with the expression cassette and targeting USP receptor expression cassette, the substance to be tested. Target expression cassette encodes a receptor polypeptide, whose expression is measured quantitatively or qualitatively, the test substance is identified as a ligand for USP thereby.