Control of gene expression in plants by receptor-mediated trans-activation in the presence of a chemical ligand

摘要

(57) [Abstract] The present invention is directed to a method of controlling gene expression in plants. Specifically, it is a method comprising obtaining a transgenic plant comprising a target at least one expression cassette and receptor expression cassettes of at least two. The first receptor expression cassette consists of a termination region and the 3 ''nucleotide sequence of the 5'' regulatory region that is operably linked to a nucleotide sequence encoding a first receptor polypeptide. The second receptor expression cassette consists of a termination region and the 3 ''nucleotide sequence of the 5'' regulatory region that is operably linked to a nucleotide sequence encoding a second receptor polypeptide. Consists termination region and the 3 ''nucleotide sequence of the 5'' regulatory region that is operably linked to a nucleotide sequence encoding a target polypeptide, the 5 ''regulatory region of the target expression cassette, the target expression cassette, the receptor where the presence of a chemical ligand of one or more complementary, are activated by the second receptor polypeptide and the first ligand binding domain of the polypeptide, to achieve expression of the target polypeptide thereby. Various properties important agronomic, for example, the method is useful for controlling plant fertility.