Process for the production of recombinant p24 proteìna and proteìna p24 recombinant human immunodeficiency vìrus.

摘要

b invention patent for the "process for the production of recombinant p24 proteu00ecna and mutants and proteu00ecna p24 recombinant human immunodeficiency vu00ecrus' ' d .The present invention relates to protein of capsu00eddio p - 24 and the production process of protein capsu00eddio p - 24 recombinant human immunodeficiency virus produced through techniques.Ngenharia genetics, to be used in the diagnosis of immunological techniques of diagnosis as well as ELISA, Western blot, etc., and a possible use as a vaccine in the prevention of infection.The DNA containing the gene coding for the protein p24 was obtained using the following steps: DNA amplification, DNA fragmentation (1), (2) the purification of DNA, digestion of DNA, (3)(4) cloning, (5) binding of DNA to the vector, (6) the transformation of competent bacteria, selection of positive clones (7), (8) the production of the protein, (9) induction of the bacteria, (10) lysis of the bacterium.(11) the purification of the protein. (12)