Process for the production of recombinant protein gp160 proteìna gp160 and recombinant human immunodeficiency vìrus.

摘要

b invention patent for the "process for the production of recombinant proteu00ecna GP 160 and proteu00ecna GP 160 recombinant human immunodeficiency vu00ecrus' ' d .The present invention relates to recombinant protein GP 160, and the production process of the protein GP 160 recombinant human immunodeficiency virus produced by techniques of engenharGoing to be employed in genetic diagnosis in various immunological techniques of diagnosis and Westem blot ELISA, etc., and a possible use as a vaccine in the preparation of the infection.The DNA containing the gene coding for the protein envelope GP 160 was performing the following steps: DNA amplification, DNA fragmentation (1), (2) DNA purification.(3) digestion of the DNA cloning (4), (5) binding of DNA to the vector, (6) the transformation of bacteria compentes, selection of positive clones (7), (8) the production of the protein, (9) induction of the bacteria.(10) lysis of the bacteria (11) purification of the protein. (12)