MUTANT PHOSPHOENOLPYRUVATE CARBOXYLASE, ITS GENE, AND PRODUCTION METHOD OF AMINO ACID.

摘要

A PHOSPHOENOLPYRUVATE CARBOXYLASE GENE, WHICH HAS MUTATION SUCH AS MUTATION TO REPLACE 625TH GLUTAMIC ACID FROM THE N-TERMINUS OF PHOSPHOENOLPYRUVATE CARBOXYLASE WITH LYSINE, MUTATION TO REPLACE 438TH ARGININE FROM THE N-TERMINUS WITH CYSTEINE AND THE LIKE, IS INTRODUCED INTO ESCHERICHIA COLI OR CORYNEFORM BACTERIA, SO AS TO PRODUCE A PHOSPHOENOLPYRUVATE CARBOXYLASE WHICH IS NOT SUBSTANTIALLY INHIBITED BY ASPARTIC ACID, THEREBY AMINO ACID IS EFFICIENTLY PRODUCED.(FIG.1)