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Regulatory sequences and expression systems functioning in filamentous fungi

机译:在丝状真菌中起作用的调控序列和表达系统

摘要

In accordance with the present invention, there is provided a promoter and terminator that function in synchronization with the expression of an endogenous gene, particularly in mycelia sterilia, in the mycelia belonging to the aprotic imperfecta. The promoter according to the present invention comprises a nucleotide sequence of the sequence number 1 and its homologue. The terminator according to the present invention comprises a nucleotide sequence of sequence number 2 and its homologue. In accordance with the present invention, there are also provided a method of producing an objective protein in a mold expressing a high expression of a target protein in a mold mold, a transformed filamentous fungus which produces high production protein and a transformed filamentous fungus.
机译:根据本发明,提供了一种启动子和终止子,其与属于非质子缺陷的菌丝体中的内源基因,特别是在菌丝体中的表达同步地起作用。根据本发明的启动子包含序列号1的核苷酸序列及其同源物。根据本发明的终止子包含序列号2的核苷酸序列及其同源物。根据本发明,还提供了在模具中产生目标蛋白高表达的模具中产生目标蛋白质的方法,产生高产量蛋白质的转化丝状真菌和转化丝状真菌。

著录项

  • 公开/公告号JPWO01/018219A1

    专利类型

  • 公开/公告日2003-04-02

    原文格式PDF

  • 申请/专利权人 明治製菓株式会社;

    申请/专利号JP特願2001-521755(P2001-521755)

  • 发明设计人 村上 健;渡辺 学;

    申请日2000-09-07

  • 分类号C12N15/09;C12N1/15;C12P21/00;

  • 国家 JP

  • 入库时间 2022-08-22 00:22:45

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