METHOD OF DETECTION OF TARGET NUCLEIC ACID SEQUENCE PRESENCE, METHOD OF DETECTION OF NUCLEIC ACID AMPLIFICATION, METHOD OF DETERMINATION OF SEQUENCE PROPERTY, METHOD OF DETECTION OF POLYMORPHISM AND/OR ALLELE VARIATION, SET

摘要

FIELD: molecular biology, biochemistry. SUBSTANCE: invention relates to methods of detection of the presence of target sequence of nucleic acid, detection of amplification of nucleic acid, determination of properties of nucleic acid sequence, detection of polymorphism and/or allele variation and discloses a set for realization of the above indicated methods also. Methods involve carrying out the amplification reaction using nucleotides labeled with fluorescent label, contact of amplification product with probe that involved reactive molecule that is able to absorb fluorescence from labeled nucleotide and fluorescence monitoring. Set for methods carrying out comprises probe containing reactive molecule, nucleotides labeled with fluorescent label and DNA-polymerase. These methods provide to detect the presence and properties of nucleic acid in sample rapidly. EFFECT: improved methods of nucleic acid analysis. 24 cl, 1 dwg, 1 tbl