Reversible binding of a fluorophore to a surface for the detection of ligat - ligand - association events by fluorescence - quenching

摘要

A process is described for the detection of ligat - ligand - association events by fluorescence - quenching in the form of a first step, the providing of a modified surface. The modification of the surface consists in the connection of at least one type of modified ligates 201, wherein the ligates 201 by a connection of at least one type of fluorophore 102 are modified, wherein the fluorophore 102 with the modified surface of a reversible binding. The further steps of the method according to the invention are providing a sample with ligands, contacting the sample with the modified surface, detection of the fluorescence of the fluorophore and comparing the detected fluorescence intensity with reference values.