Reversible binding of a fluorophore to a surface for the detection of ligat - ligand - association events by fluorescence - quenching

摘要

A method for the detection of ligat - ligand - association events by fluorescence - quenching comprising the steps ofa) providing a modified surface, wherein the modification in the connection of at least one type of modified ligates (201) and wherein the ligates (201) by a connection of at least one type of fluorophore (102) are modified, wherein the fluorophore with the modified surface of a reversible binding,b) providing a sample with ligands,c) contacting the sample with the modified surface,d) detection of the fluorescence of the fluorophore (102),e) in step d) comparing the detected fluorescence intensity with reference values.