REVERSIBLY BINDING A FLUOROPHORE TO A SURFACE FOR THE PURPOSE OF DETECTING LIGATE/LIGAND ASSOCIATION EVENTS BY FLUORESCENCE QUENCHING

摘要

The invention relates to a method for detecting ligate/ligand association events by fluorescence quenching, which comprises as a first step the provision of a modified surface. The modification of the surface consists in the binding of at least one type of modified ligates 201, wherein said ligates 201 are modified by at least one type of fluorophore 102 bound to it, said fluorophore 102 establishing a reversible bond with the modified surface. The further steps of the inventive method are: providing a sample that includes ligands, contacting the sample with the modified surface, detecting the fluorescence of the fluorophore and comparing the detected fluorescence intensity with reference values.