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《中国化学快报:英文版》
>A novel detection of single-stranded DNA binding protein based on ss-DNA modified chip using surface plasmon resonance microscopy
A novel detection of single-stranded DNA binding protein based on ss-DNA modified chip using surface plasmon resonance microscopy
An ss-DNA gold chip was prepared based on self-assembly of the thiol-derivatized oligonucleotide,and used for thedetermination of single-stranded binding protein(SSB)by surface plasmon resonance microscopy(SPR).The experiment resultsshowed that SSB binds ss-DNA with high specificity,and relative signal of SPR response is proportional to the concentration of SSBin the range of 0.1-100 ng/mL with a detection limit(S/N=3)of 0.07 ng/mL.
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