Sepn. of chymosin from liq. contg. pepsin

摘要

Method for separating chymosin from a liq. contg. the milk-clothingenzymes chymosin and pepsin comprises: adjusting the liq. to pH 3.8-5.2 and conductivity 2,000-19,000 micromho; equilibrating an anionic exchange medium to this pH and conductivity range; contacting the liq. with the medium to bind pepsin tothe medium; recovering chymosin in the treated liq., and periodically contacting the exchange medium with a soln. for removing the bound pepsin.