PLASMID TO BE USED IN CHROMOSOME RECOMBINATION OF ESCHERICHIA COLI

摘要

A technique of efficiently and artificially recombining a specific gene on the chromosome of a microorganism belonging to the genus Escherichia which is highly useful as a bacterium producing various useful substances. A plasmid, which is replicable in Escherichia coli K-12 strain but non replicable in microorganisms belonging to the genus Escherichia other than the Escherichia coli K-12 strain, is constructed. A target gene is inserted into this plasmid and then transferred to a microorganism belonging to the genus Escherichia other than the Escherichia coli K-12 strain. Thus, a strain having the substitution of the target gene can be efficiently selected.