A film holder includes a base, a cover, and a flattening mechanism. The base has a film panel for receiving a film and a plurality of sliding slots formed at its two opposite sides. The cover is mounted on the base and is slidable on the base in a first direction, and at least one side of the cover is provided with a recess. The flattening mechanism is fit on the recess to flatten the film on the base by the slide of the cover in the first direction after the cover is mounted on the base. The flattening mechanism includes a sliding rod, at least one resilient member, a contact plate, and a plurality of cylindrical parts formed on the bottom of the sliding rod.
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