DEVELOPMENT OF MOLECULER MAKER FOR MEAT QUALITY AND CHARACTERISTICS BREED IN HANWOO

摘要

The invention of DNA from the progeny produced from beef steers groups Sires candidate after separation and purification, located in exon 2 After the two kinds of bovine leptin gene nucleotide sequence of the primer by synthesis of a second locus having a specific nucleotide sequence and PCR performed using a PCR-product 2 kinds of restriction enzymes was reacted with Kpn2 I, Msp I alleles was analyzed in the form of meat quality and relevance to black. Using the frequency of the leptin gene obtained from the PCR-RFLP can identify the characteristics of the beef population. TT genotype of Kpn2 I showed statistical significance in relation to the meat. Using the expression frequency of the genotype by PCR-RFLP analysis to identify the genetic characteristics of the beef, and can obtain a large effect in improving the TT genotype using a molecular marker for beef meat quality improvement.