MUTANT STRAIN OF HEPATITIS B VIRUS AND ITS USING

摘要

FIELD: biotechnology, medicinal virology, medicine.;SUBSTANCE: invention describes isolated strain of human hepatitis B virus "S"-133 Ooh comprising genome of subtype adr that is deposited in the European collection of cellular cultures at № P 97121504 or P 97121505 or P 97121506 and encoding polypeptide of basic surface antigen of human hepatitis B virus. Abovementioned antigen comprises mutation in amino acid sequence (polypeptide), namely, methionine at 133 position is replaced with threonine. Invention discloses variants of isolated nucleic acids encoding the mutated polypeptide and variants of isolated nucleic acids encoding peptides showing properties of the indicated polypeptide. The description text gives nucleotide sequences of nucleic acid variants encoding abovementioned polypeptide and peptides. Also, invention describes variants of vectors used in cloning DNA fragments of mutant human hepatitis B virus comprising isolated nucleic acid and isolated nucleic acid bound functionally with RNA transcription promoter respectively and wherein each isolated nucleic acid encodes indicated polypeptide. Invention discloses methods for producing polypeptide and peptide and methods for preparing the purified polypeptide and peptide by using indicated vectors. Also, invention describes purified polypeptides and peptides of mutated antigen retaining antigenic properties of polypeptides of the natural human hepatitis B virus, and methods for preparing antibodies raised to them and anti-antibodies, among them, monoclonal antibodies. Also, invention discloses different methods for using abovementioned isolated nucleic acids, polypeptides and peptides for aim for diagnosis and treatment of diseases associated with human hepatitis B virus. Using the proposed invention provides the development of diagnosis schedules and treatment of diseases associated with the mutated human hepatitis B virus.;EFFECT: valuable medicinal properties of mutant virus.;66 cl, 2 dwg, 4 ex