A PROCESS FOR DIFFERENTIATING A NEURAL PROGENITOR CELL TO A DOPAMINERGIC NEURAL CELL AND A CULTURE MEDIUM THEREFOR

摘要

A process for differentiating a neural progenitor cell to a dopaminergic neural cell, and a culture medium therefor are provided to improve differentiation efficiency from a mesenchymal stem cell to the dopaminergic neural cell by using specific medium. The process for differentiating the neural progenitor cell to the dopaminergic neural cell comprises the steps of: (a) culturing the neural progenitor cell in N2 medium containing 20-100 ng/ml of fibroblast growth factor 8(FGF 8), 200-500 ng/ml of sonic hedgehog(SHH), 100-300 muM of ascorbic acid(AA) and 10-20 ng/ml of brain-derived neurotrophic factor(BDNF); and (b) culturing the cultured product in N2 medium containing 100-300 muM of ascorbic acid(AA), 10-20 ng/ml of brain-derived neurotrophic factor(BDNF), 2-20 ng/ml of glial cell-derived neurotrophic factor(GDNF) and 0.5-2 mM of dibutyryl cyclic adenosine monophosphate(dcAMP).