A PROCESS FOR DIFFERENTIATING A NEURAL PROGENITOR CELL TO A CHOLINERGIC NEURAL CELL AND A CULTURE MEDIUM THEREFOR

摘要

A process for differentiating a neural progenitor cell to a cholinergic neural cell, and a culture medium therefor are provided to differentiate adult stem cells containing mesenchymal stem cell derived from cord blood into the cholinergic neural cell in vitro, and improve differentiation rate from the neural progenitor cell to the cholinergic neural cell. The process for differentiating the neural progenitor cell into the cholinergic neural cell comprises the steps of: (a) culturing the neural progenitor cell in N2 medium containing 50-500 ng/ml of sonic hedgehog(SHH) and 0.1-2 muM of retinoic acid(RA); (b) culturing the culture product of step(a) in N2 medium containing 1-20 ng/ml of basic fibroblast growth factor(bFGF) and 50-500 ng/ml of sonic hedgehog(SHH); and (c) culturing the culture product of step(b) in N2 medium containing 100-300 muM of ascorbic acid(AA) and 10-20 ng/ml of brain-derived neurotrophic factor(BDNF), wherein the neural progenitor cell is obtained from the mesenchymal stem cell derived from cord blood.