A PROCESS FOR THE PREPARATION OF MOLECULARLY IMPRINTED POYMERS USEFUL FOR SEPARATION OF ENZYMES

摘要

This invention relates to the process for the preparation of molecularly imprinted polymers useful for separation of enzymes. Molecularly imprinted polymers prepared by the process of the present invention exhibit selective binding of imprinted enzyme which is useful in the imprinted enzyme form aqueous solution of the imprinted enzyme or a mixture containing imprinted enzyme and other enzymes. Process steps comprises; reacting the complex of enzyme and affinity monomer, a co monomer and a cross linker with a polymerization initiator and a polymerization accelerator at ambient temperature and pressure for a period ranging between 2 to 24 hrs, to obtain a cross linked polymer, crushing the said cross linked polymer to fine particles, adding a solvent and extracting imprinted enzyme from the cross linked polymer to obtain the molecularly imprinted polymer.