Flavone synthase derived from Oriza sativa flavone synthase gene methods for preparing the same and process for producing apigenin in microorganism by using the same

摘要

isoflavone synthase isolated from The invention of rice ( Oriza sativa ) ( flavone synthase;. FNS), relates to the production method of the his gene and their manufacturing method and the flavone Bahia using microorganisms expressing synthase angiogenin (apigenin) ; In detail, the present invention provides a more Naryn angiogenin (naringenin) from Bahia flavone synthase producing angiogenin, his genes and their production method, wherein the flavone synthase to produce a recombinant expression vector and this transformed E. coli, and apigenin using said transformed E. coli and relates to a method of mass production, the production method of the flavone apigenin, such as using a synthase gene of the present invention can be industrially widely applied from a very economical and effective way to obtain large quantities of high value-added substrate metabolite.