Methyltransferses derived from plants methyltransferase genes methods for preparing the same and process for producing ermanin 57-dihydroxy-34'-dimethylflavone in microorganism by using the same

摘要

The invention of methyl transferase derived from plants (o-methyltransferases), their genes and the methyl the recombinant expression vector to produce the transformed microorganism with the enzyme and the transition, the present invention relates to a production method of the production method of these multi-methylation using methyl transferase and using the same (multiple methylation) is a metabolite. ; than More specifically, the present invention is rice with each other, and selective separation of the other methyl transferase from soybean, their genes, the methyl transferase gene in a recombinant expression vector comprising one or more, in which E. coli transformed, using the same method for producing a methyl transferase, and non-IRMA (ermanin; 5,7-dihydroxy-3,4-dimethylflavone) using microorganisms which multiple expressing the methyl transferase relates to a method of mass production, methyl transferase of the present invention However, the method is useful for chemical synthesis is more easily mass-produced at low cost through a very difficult, because the metabolic biotransformation reaction product can be widely used in new drug development.