A genetic marker for race-typing of Xanthomonas oryzae pv. oryzae is provided to reduce the time and labor of race-typing, and appropriately control the rice disease caused by a specific race of Xanthomonas oryzae pv. Oryzae through accurate identification of the race from races K1, K2, K3, K4 and K5. A genetic marker for race-typing of Xanthomonas oryzae pv. oryzae has the nucleotide sequence of SEQ ID NO:1. A method for race-typing of Xanthomonas oryzae pv. oryzae comprises the steps of: (a) isolating the genomic DNA from Xanthomonas oryzae pv. oryzae; (b) digesting the genomic DNA with restriction enzymes EcoRI and HindIII; (c) separating the digested genomic DNA fragments in their size; (d) hybridizing the separated genomic DNA fragment with the genetic marker as a probe; (e) detecting the hybridized probe; and (f) analyzing the pattern of detected probe, wherein the probe is obtained by performing PCR(polymerase chain reaction) of the genomic DNA from Xanthomonas oryzae pv. oryzae using primers of SEQ ID NO:2 and SEQ ID NO:3.
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机译:米氏黄单胞菌pv种族分型的遗传标记。提供稻米以减少种族分型的时间和劳力,并适当地控制由稻黄单胞菌的特定种族引起的水稻疾病。通过准确识别来自K1,K2,K3,K4和K5种族的Oryzae。米氏黄单胞菌pv种族分型的遗传标记。稻米具有SEQ ID NO:1的核苷酸序列。一种米黄单胞菌PV的种族分型方法。稻米包括以下步骤:(a)从稻黄单胞菌PV分离出基因组DNA。水稻(b)用限制酶EcoRI和HindIII消化基因组DNA; (c)按大小分离消化的基因组DNA片段; (d)将分离的基因组DNA片段与遗传标记作为探针杂交; (e)检测杂交探针; (f)分析检测到的探针的模式,其中所述探针是通过对来自米黄单胞菌(Xanthomonas oryzae pv)的基因组DNA进行PCR(聚合酶链反应)而获得的。使用SEQ ID NO:2和SEQ ID NO:3的引物形成米。
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