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Piglet production method by simultaneous transfer of embryos formed by in vitro fertilization and nuclear transfer

机译:通过体外受精和核移植同时移植胚胎的仔猪生产方法

摘要

A method for producing piglet is provided to be able to increase the normal birth rate of cloned pig by co-transplantation of in vitro fertilization and somatic cell cloned fertilized eggs, significantly shorten the culturing period of the fertilized egg and improve the normal birth rate from a small number of ova. A method for producing piglet comprises the steps of: (a) culturing collected immature ova of slaughtered pig; (b) after preparing (i) a fertilized egg obtained by in vitro fertilization of the mature ova prepared by the step(a) and liquid semen, and (ii) a somatic cell transplanted fertilized egg obtained by transplanting a donor somatic cell into an enucleated prepared mature ova prepared by enucleating the mature ova of the step(a), respectively culturing (i) and (ii) for 16-36 hours; and (c) transplanting 150-250 fertilized eggs prepared by the in vitro fertilization and the somatic cell transplantation into one surrogate mother. Further, a culture ground of the immature ova contains 2-6 follicle films having 4-6 mm of size.
机译:本发明提供了一种仔猪的生产方法,其能够通过体外受精和体细胞克隆的受精卵的联合移植来提高克隆猪的正常出生率,显着缩短受精卵的培养时间并提高从少量的卵。一种生产仔猪的方法,包括以下步骤:(a)培养收集的屠宰猪的未成熟卵; (b)制备(i)通过对步骤(a)制备的成熟卵和精液进行体外受精而获得的受精卵,以及(ii)通过将供体体细胞移植到体通过将步骤(a)的成熟卵去核,分别将(i)和(ii)培养16-36小时来制备去核的制备的成熟卵。 (c)将通过体外受精和体细胞移植制备的150-250个受精卵移植到一位代孕母亲中。此外,未成熟卵的培养基包含大小为4-6mm的2-6个卵泡膜。

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