Improved method for detecting ATP.

摘要

A method for detecting ATP in a sample comprising: (a) adding to the sample a reagent composition comprising one or more detergents and a luciferase comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 1, 2, 3 and 4 wherein the reagent composition is capable of maintaining at least 30% activity, as measured by luminescence after the reagent composition is combined with the sample, for at least one hour compared to the activity of the reagent composition immediately after the luciferase is combined with the one or more detergents, and wherein the one or more detergents present in the reagent composition are selected from cationic detergents, anionic detergents and / or zwitterionic detergents and are collectively able to reduce ATPase activity endogenous to the sample by at least 25% relative to the ATPase activity in the absence of one or more dete rgentes and (b) detecting luminescence.