Method of determining oxidation stability of lipids in muscle tissue

摘要

The invention relates to the determination of oxidation stability of lipids in samples of muscle tissue, which is first homogenised sample of muscle tissue, then extracted with an aqueous solution of trichloroacetic acid, followed by filtration of the extract sample through a paper and a membrane filter, then reacted clarified extract with a solution of thiobarbituric acid for formation of a colored complex when heated in a water bath at 100 degC, and the resulting colored complex spectrophotometrically analyze against the blank and according to the calculated content of malonaldehyde in mg / kg down lipid oxidation stability of the sample of muscle tissue. The new method is faster and cost-effectiveness while maintaining sufficient sensitivity and accuracy of the assay. It has a great use in monitoring the impact of the composition of animal feed on meat quality changes during storage.