IDENTIFICATION OF NOVEL CELL SURFACE MARKERS FOR PANCREATIC PROGENITOR CELLS AND DEFINITE ENDODERMAL CELLS

摘要

Provided are methods of identifying, isolating and qualifying pancreatic progenitor cells and definite endodermal cells. Also provided are an isolated population of pancreatic progenitor cells, comprising at least 75% of cells having a TROP-2+ and/or TROP-2+/GPR50+ expression pattern and an isolated population of definite endodermal cells, comprising at least 50% of cells having a SOX17+/SOX7+/GSC+/CER+/FOXA2+/CXCR4+/NANOG expression pattern. Also provided are nucleic acid constructs comprising a reporter protein under the transcriptional regulation of SOX17 regulatory sequence or of PDX1 regulatory sequence, and cells comprising same, and methods and kits using same.