In-situ Quantitative Analysis Method of Enzyme Reaction using AFM, and AFM Cantilever and AFM System for the In-situ Quantification Analysis

摘要

The present invention is how real-time quantitative analysis of the enzyme reaction using the AFM, according to the AFM cantilever and AFM systems for the analysis, and immobilized enzyme substrate to the AFM cantilever surface for, treating the enzyme substrate with the enzyme, the enzyme substrate and wherein the real-time quantitative analysis of the according to the reaction between the enzyme comprising the step of measuring a resonance frequency shift of the cantilever in real time, the enzyme reaction using the AFM method, according to AFM system including AFM cantilever for for the analysis and this will be. ; Cantilever, an enzyme, a peptide, a real-time, quantitative analysis, a resonance frequency, AFM