Method of production of restorer lines of Brassica napus double zero with a good agronomic value

摘要

Method of producing double zero Brassica napus for masculinacitoplásmica sterility (cms) Ogura having introgression carrier radish restorer gene Rfo suppressed delalelo Pgi-2 radish and recombined with the Pgi-2 gene from Brassica oleracea restorer lines, and have good valoragronómico characterized by female fertility, a good transmission rate of Rfo and vegetativoelevado force, said method including the steps of: a) crossing the cms lines double zero Brassica napus spring comprising a suprimidade insertion radish each side of Rfo, having lost such double zero Brassica napus deprimavera expression isoenzyme Pgi-2 allele radish genome and of Pgi-2 allele of the genome deB emc lines. oleracea, with the double zero Drakkar spring to form restored heterocigóticasde Brassica napus, b) irradiating before meiosis the restored plants heterozygous obtained in step a) with derayos gamma irradiation plants, c) crossing pollen from flowers line obtained in step b) with the spring line Wesroona rms double zero, d) testing the progeny for vigor, female fertility and transmission rate of the cms gene, e) selecting progeny lines.