Process for Preparing Fibrinolytic protease from Streptomyces sp. CS684

摘要

The present invention relates to a thrombolytic enzyme, and a method of producing the derived Streptomyces spp., Fibrin dissolved enzyme according to the present invention is isolated from the genus Streptomyces strain (Streptomyces sp. CS684) (accession No. KCTC 10631 BP) , purification of the enzyme SDS-PAGE and fibrin-party has a molecular weight of about 35 kDa by our aunt, the maximum activity appeared in 45 ℃ and pH 7-8, and pH 6-9 at a temperature stability of 40 ℃, and the fibrinogen Bβ - one chain hydrolysis, Aα-and γ - chain does not cut, the azo-casein for K m, V max and K cat value was ^{4.2 mg ml -1, 305.8 μg min} -1 mg -1 and 188.7 s ^-1, and the activity of Cu ²⁺ and Fe ²⁺ is suppressed by 90% or more in 2mM levels, protein activity is not inhibited by aprotinin and PMSF, 50% of the pepper 4mM by block (pefabloc) or more is suppressed, and characterized in that more than 80% inhibition at 1mM is by EDTA and EGTA, can be used as a thrombolytic agent because of the excellent resolution of fibrin. ;