PURPOSE: Provided are the nerve cell protectants produced from Streptomyces sp. and preparation method thereof. In vitro test, their pharmaceutically acceptable organic and inorganic salts, hydrates and isomers show strong inhibition of the cell toxicity and thereby protect the nerve cells. CONSTITUTION: The derivatives isolated from the growth medium of Streptomyces sp. genus 833 (KCTC 8796 P), are represented by the chemical structure 1, 2, 3. These phenazostatin structures are identified by the instrumental analysis. These compounds are separated from the organic solvents extracts of the growth medium through column chromatography and final 3 active fractions are recrystallized from methanol. In screening, N18-RE-105 cell has been used as the vitro model to examine the compounds. In the test, the compounds show inhibitory activity against the active oxygens such as superoxide, hydrogen peroxide and hydroxyl radical. These phenazostatin compounds show strong inhibition activity of L-glutamate cell toxicity of the above model cell.
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