METHOD OF DETERMINING SENSITIVITY OF MICROORGANISMS OF Salmonella GENUS TO ANTIBACTERIAL AGENTS

摘要

FIELD: biotechnology.;SUBSTANCE: test kind of microorganisms of the genus Salmonella is incubated, which is taken at a final concentration of approximately 1000 cells/ml, with the test antibacterial agent in the known concentration previously specified for each antibacterial agent, which is optimal for exposure to the specified kind of microorganisms. Incubation is carried out at a temperature of 37°C for 24 hours. The samples are added to the aptamers specific to test species of living microorganisms, fluorescently labelled, at a final concentration of 100 nM. It is incubated for the second time for 20 minutes. The sample is examined using flow cytometry. The level of fluorescence of aptamers is determined by graphs made with a flow cytometer using a program which enables to display a percentage value. The part of bound and un-bound bacteria with the aptamers fluorescently labelled is determined. The amount of the living or non-living organisms in the sample is determined.;EFFECT: method enables to determine with the high degree of accuracy the sensitivity of microorganisms to antibacterial agents.;2 dwg, 1 ex