METHOD FOR DETERMINING THE SENSITIVITY OF MICROORGANISMS TO CHEMICAL AGENTS

摘要

The invention relates to a method for determining the sensitivity of microorganisms to chemical agents or antibiotics, which consists in the fact that the microorganisms are cultured in a liquid nutrient medium with a chemical agent or antibiotic, then the medium is treated in such a way that cell lysis and distribution of microorganism fragments by volume of the medium can occur, and then, in the microorganism-free supernatant, the content of protein components is measured and the values are compared with control or standard samples. The method involves adding a certain amount of microbial cells to a liquid nutrient medium, into which an enhancing agent specific for a specific group (genus or family) of microorganisms is added. The liquid nutrient medium containing microorganisms is preincubated, and then, after the addition of a certain amount of antibiotic, it is again incubated. Then the cells are removed from the incubation medium to obtain a supernatant. The supernatant proteins are extracted by the addition of alcohol, followed by the isolation of proteins. Protein precipitate is used to obtain a mass spectrum used for comparison with standard mass spectra.