For nucleic acid amplification that is initiated at a high temperature, and chemically modified nucleoside 5'-triphosphate

摘要

As used herein, the methods and compositions for amplifying nucleic acid is provided. In a nucleic acid amplification reaction, these methods involve the use of a replacement terminated primer or 3 'substituted nucleoside 5'-triphosphate,' 3. In certain embodiments, the method is achieved by using lead utility in nucleic acid amplification, a primer termination substitution and / or 3 '-substituted NTP' 3. In a preferred embodiment, the 3 'position, and the primers or NTP / are substituted and carbonic ester ethers, esters, or by chemical groups thermolabile particular.