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Chemically modified nucleoside 5'-triphosphates for thermally initiated amplification of nucleic acid
Chemically modified nucleoside 5'-triphosphates for thermally initiated amplification of nucleic acid
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机译:化学修饰的5'-三磷酸核苷,用于热引发核酸扩增
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摘要
Provided herein are methods and compositions for nucleic acid amplification. These methods involve the use of 3'-substituted nucleoside 5'-triphosphates or 3'-substituted terminated primers in nucleic acid amplification reactions. In certain aspects, the methods are accomplished by use of 3'-substituted NTPs and/or 3'-substituted terminated primers which provide utility in nucleic acid amplification. In preferred embodiments, the NTPs and/or primers are substituted at the 3'-position with particular heat labile chemical groups such as ethers, esters or carbonate esters.
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