Dna based diagnostics for identification of citrus rootstock cultivars

摘要

Rootstocks usages in perennials are prominent as also in citrus. Rangapur lime, Rough lime and Galgal are three important rootstocks used in citrus nursery industry. Selection of preferred rootstocks is therefore important in citrus industry and most of the citrus orchards need to validate rootstocks types due to use of galgal rootstocks. The present disclosure provides two genomic loci specific SCAR primers developed from RAPD and SRAP primers for identification and differentiation of citrus rootstocks. The present disclosure also relates to method of identifying Citrus DNA sequence as well as PCR reaction mixtures and kits for identification and amplification of Citrus DNA sequences and certifying the rootstock used in citrus industry.