Method for preparation of a strain of evolved micro-organism for the production of 1,2-propanediol from a carbon source, said method comprising: - growing an initial strain under selection pressure in an appropriate growth medium, said strain comprising bacterial attenuation of the expression of the tpiA gene and an attenuation of the expression of at least one of the genes involved in the conversion of methylglyoxal into lactate, in order to promote the evolution of said initial strain, - selecting and isolating the evolved strain having an increased production rate of 1,2-propanediol, - reconstruct a functional tpi gene in the evolved strain.
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