Primer preparation method, primer usage method, primer set, PCR reaction solution, and primer design method

摘要

PROBLEM TO BE SOLVED: To produce a primer set for PCR (polymerase chain reaction) that can simultaneously and specifically amplify a plurality of detection object organisms.SOLUTION: The primer set used for amplifying the base sequence of DNA by a PCR method is produced such that at least one base sequence of a forward primer and a reverse primer for amplifying an amplification object region in DNA of an amplification object organism does not overlap with the base sequence of DNA of a non-amplification-object organism continuously by 12 bases or more.