METHOD FOR ASSESSING SUGAR BEET BREEDING MATERIAL FOR COLD STRESS RESISTANCE IN CULTURE IN VITRO

摘要

A method for assessing sugar beet breeding material for cold stress resistance in culture in vitro includes: use flowering shoots or inflorescenses as initial explants, introducing the initial explants in culture in vitro, under conditions of cooling in a thermal chamber at + 2-4 °C. Used are different kinds of explants, in particular seeds and axillary buds; buds in vitro are the object of evaluation for cold stress resistance; duration of cold stress - 1-2 months; the resistance character is determined by the ability to restore the growth processes of buds and by the determined multiplication coefficient after 6 weeks of cold treatment.