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PREPARATION METHOD FOR BACILLUS COAGULANS BACTERIAL SUSPENSION

机译:芽孢杆菌凝集菌悬浮液的制备方法

摘要

The invention relates to a preparation method for a Bacillus coagulans bacterial suspension. The method comprises: inoculating a Bacillus coagulans CGMCC No. 6681 strain into a flat plate of a solid culture medium for activating bacteria, then carrying out seed culture and fermentation culture, finally centrifuging the fermented liquid obtained by the fermentation culture, removing supernatant, taking bacterial sludge, blending with sterile water to prepare a 5×1010 to 8×1010 cfu/mL bacterial suspension, and then adding a surfactant to obtain the Bacillus coagulans bacterial suspension. The colonization capacity of bacterial suspension prepared by the method of the invention is enhanced in plant body surface, and growth promotion, bacterium inhibition and insect killing are integrated. The multiple effects of the bacterial suspension determine that the bacterial suspension has great prospect in popularization and meets the requirements of modern agriculture.
机译:本发明涉及一种凝结芽孢杆菌细菌悬液的制备方法。该方法包括:将凝结芽孢杆菌CGMCC No.6811菌株接种到用于活化细菌的固体培养基的平板中,然后进行种子培养和发酵培养,最后将通过发酵培养得到的发酵液离心分离,除去上清液,取入。细菌污泥,与无菌水混合以制备5×10 10 至8×10 10 cfu / mL细菌悬浮液,然后添加表面活性剂以获得凝固芽孢杆菌细菌悬挂。通过本发明的方法制备的细菌悬浮液的定殖能力在植物体表面中增强,并且整合了生长促进,细菌抑制和杀虫作用。细菌悬浮液的多重作用决定了该细菌悬浮液具有广泛的应用前景,符合现代农业的要求。

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