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外文期刊>药物分析学报:英文版
>Preparation of recombinant firefly luciferase by a simple and rapid expression and purification method and its application in bacterial detection
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Preparation of recombinant firefly luciferase by a simple and rapid expression and purification method and its application in bacterial detection
A simple and rapid expression and purification method of recombinant firefly luciferase was developed for bacteria detection. A modified luciferase gene from North American firefly Photinus pyralis was cloned into pET28a expression vector and the recombinant protein was produced in Escherichia coli BL21. The recombinant luciferase,equipped with a polyhistidine affinity tag,was purified by immobilized metal ion affinity chromatography (IMAC). The approach generated an abundant expression and an efficient pur...
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